16S rRNA gene for ribotyping Brucella: A monospecific genus
نویسندگان
چکیده
منابع مشابه
Use of 16S rRNA gene sequencing for rapid confirmatory identification of Brucella isolates.
Members of the genus Brucella are categorized as biothreat agents and pose a hazard for both humans and animals. Current identification methods rely on biochemical tests that may require up to 7 days for results. We sequenced the 16S rRNA genes of 65 Brucella strains along with 17 related strains likely to present a differential diagnostic challenge. All Brucella 16S rRNA gene sequences were de...
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Misidentification of Brucella species from clinical specimens using commercial bacterial identification systems is a recurring problem. An isolate from a bacterimic patient was identified as Bergeyella zoohelcum by MicroScan Walk-Away (Siemens Healthcare Diagnostics Inc., West Sacramento, CA, USA) and as Brucella melitensis by Vitek 2 system (bioMérieux Inc., Durham, NC, USA). Because of this i...
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The inclusivity, exclusivity, and detection limit of six 16S rRNA gene-based Helicobacter genus-specific PCR assays were examined. Five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). The clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture.
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A method is described in detail for the preparation of monospecific sera for typing brucella cultures. The importance of using entirely smooth cultures throughout the procedure is emphasized. A single intravenous injection of living brucellae in rabbits caused an active infection and the early appearance of high-titre antibodies. Sera obtained five to seven days after inoculation contained a hi...
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Uncovering the complexities of trophic and metabolic interactions among microorganisms is essential for the understanding of marine biogeochemical cycling and modelling climate-driven ecosystem shifts. High-throughput DNA sequencing methods provide valuable tools for examining these complex interactions, although this remains challenging, as many microorganisms are difficult to isolate, identif...
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ژورنال
عنوان ژورنال: VETERINARY SCIENCE RESEARCH JOURNAL
سال: 2014
ISSN: 0976-1284,2230-942X
DOI: 10.15740/has/vsrj/5.1and2/22-26